01 / RESEARCH SUMMARY
PE-22-28 Research Summary
PE-22-28 is a synthetic analog of spadin, a peptide derived from the sortilin protein. It blocks TREK-1 potassium channels in the brain, a target linked to depression. In mouse studies, it produced antidepressant-like effects within days rather than weeks and increased the growth of new neurons in the hippocampus. It has not been studied in humans.
Read the full guide →02 / WHY RESEARCHERS STUDY IT
Why PE-22-28 draws research interest
These are the areas researchers and the peptide community explore PE-22-28 for most often. They describe what is being studied — not proven benefits, approved uses, or promised results.
Mood
Produced fast antidepressant-like effects in mice.
Neurogenesis
Increased new neuron growth in the hippocampus.
TREK-1 target
Blocks a potassium channel linked to depression.
Evidence ranges from early lab work to large clinical trials depending on the use — see the sections below for the data and sources.
03 / DOSING & RECONSTITUTION
Dosing & Reconstitution Guide
A single practical dilution with accurate dosing, step by step
Standard Approach (3 mL = 3.33 mg/mL)
- Reconstitute: Add 3 mL bacteriostatic water to one 10 mg vial → final concentration 3.33 mg/mL (3,333.3 mcg/mL).
- Typical range: 50–200 mcg 1x daily · daily.
- Easy measuring: At 3.33 mg/mL, 1 unit ≈ 33.33 mcg on a U-100 syringe, so doses read at 1.5–6 units — a 0.3 mL (30-unit) syringe makes them easier to read.
- Storage: Lyophilized: store at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and do not freeze the mixed solution.
| Protocol | Dose range | Frequency | Half-life | Fasted | Route |
|---|---|---|---|---|---|
| Injectable · Subq | 50–200 mcg | 1x daily · Daily | ~2–4 hrs | No | Subq |
04 / RECONSTITUTION CALCULATOR
Mix & measure PE-22-28 · 10 mg
Pre-filled with this protocol's recommended BAC water and starting dose — edit any field to run your own numbers.
- CONCENTRATION
- 3.33 mg/mL
- DRAW VOLUME
- 0.015 mL
- ON THE SYRINGE
- 1.5 units
- DOSES / VIAL
- 200
Reconstitution math only. U-100 syringe: 100 units = 1 mL.
Reconstitution GuideFull calculator →05 / STORAGE
Storage Instructions
Lyophilized (powder)
Store in the freezer at −20 °C (−4 °F) for long-term storage, or refrigerated at 2–8 °C for shorter periods. Keep dry, sealed and away from light. Let a frozen vial reach room temperature before opening to avoid condensation.
Reconstituted
Refrigerate at 2–8 °C (35.6–46.4 °F) and use within about 28 days. Do not freeze. Keep away from heat and direct light, and avoid repeated temperature swings. Swirl gently — never shake.
Reconstitution steps
- Wipe the vial stopper with an alcohol swab.
- Draw the BAC water and release it slowly down the inside wall of the vial.
- Swirl or roll gently until fully dissolved — don't shake.
- Label with the date and concentration and refrigerate.
06 / RESEARCH FINDINGS
What Research Shows
Preclinical / limited human evidence
- TREK-1 potassium-channel activity and antidepressant-like effects have been explored preclinically.
Research context
Animal behavioral outcomes do not establish antidepressant effectiveness or safety in humans.
07 / MOLECULAR BIOLOGY
Mechanism of Action
Investigational mechanism
TREK-1 potassium channel
A spadin-derived peptide investigated for inhibition of TREK-1 and antidepressant-like effects in preclinical work.
Adamantane & the blood–brain barrier
Chemically attaching an adamantane group to a peptide can increase its lipid (fat) solubility. Researchers hypothesize that this modification may help certain compounds cross the tightly regulated blood–brain barrier. The effect depends on the specific modified molecule; greater lipid solubility alone does not establish brain delivery or clinical benefit. This does not apply automatically to the unmodified peptide and is not a reconstitution additive.
08 / LIFESTYLE FACTORS
Lifestyle Factors
- Cycle it: run on/off cycles to maintain responsiveness.
- Timing: dose stimulating peptides (Semax family) early in the day; calming ones (Selank, DSIP) later.
- Sleep and hydration have a bigger effect on cognition than any peptide.
- Limit caffeine stacking to avoid overstimulation.
09 / BENEFITS & SIDE EFFECTS
Potential Benefits & Side Effects
Potential Benefits
- Improved focus and mental clarity
- Reduced anxiety and stress reactivity
- Better mood and motivation
- Neuroprotective support (BDNF-related peptides)
Side Effects
- Nasal irritation with intranasal use
- Mild headache
- Overstimulation or irritability (Semax-type)
- Drowsiness (Selank, DSIP)
10 / STUDIES
Studies & references
11 / SAFETY
Safety considerations
- Verify the active identity, lot number, and matching certificate of analysis. Testing does not by itself establish sterility or suitability for human use.
- Follow documented product-specific storage and preparation requirements; stability and diluent compatibility depend on the formulation.
- Research powders are not interchangeable with prescription formulations. Do not infer an injection or nasal protocol from a concentration calculation.
- For medical care, pregnancy, existing conditions, or medication interactions, consult a qualified healthcare professional.
12 / RECOMMENDED SOURCE
Recommended Source
For research peptides, we point researchers to Orbitrex Peptides for PE-22-28.
Why Orbitrex?
- Family-owned & independently tested: Multiple laboratories verify quality, with batch-specific testing in every COA.
- Everyday value: Buy 4, Get 1 Free on eligible products.
- Loyalty credit: 5% back in loyalty credit for future purchases.
- VerifyPass discount: 20% off for qualifying groups — stacks with affiliate codes.
- Free 2nd Day shipping: Free UPS 2nd Day Air on orders $200+, applied automatically at checkout.
All products are for research use only. Availability and offers subject to change.